Question

Do you need to heat a solution in order for protein to be detected using a Biuret Test?

Answer 1

rate of reaction increases upon heating

Explanation:

Your Doubt is correct it does speed up the reaction as you increases the temperature due the fact that formation of biuret the compound is accelerated in the presence of heat. which then forms the coordinated compound with the cupric (Cu2+) ion.

Answer 2

It does not need to be heated.

Explanation:

Biuret solution will cause a solution which contains protein to turn purple. The reaction will take place almost instantly, even if the protein solution is near room temperature.

This video was shot testing solutions which were at room temp. You can see how quickly the color change occurs as drops of Biuret reagent are added.

Hope this helps!