What is southern blotting?

1 Answer
Linica Uday
Aug 9, 2017

Southern blotting is a method used in molecular biology for detection of a specific DNA sequence in DNA samples.

Explanation:

It is the transfer of DNA fragments from an electrophoresis gel to a membrane support, resulting in immobilisation of the DNA fragments. The membrane carries a semipermeable reproduction of the banding pattern of the gel.

The steps involved are:
1) DNA digestion
2) Gel electrophoresis
3) Blotting.
4) Probe labelling
5) Hybridisation and washing
6) Detection

Southern blotting analysis reveals information about DNA identity, size and abundance.

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