Characterize adenine using #"UV-Vis"# (ultraviolet-visible), #"IR"# (infrared), #"MS"# (mass spectroscopy), and #""^(13) "C"# #"NMR"# (nuclear magnetic resonance) data?
Warning! Very long answer!
For reference, here is the structure of adenine.
The UV spectrum measures the absorption of energy in the 200 nm to 400 nm region as nonbonding or
The spectrum is a plot of the intensity of the absorption against the wavelength.
The peaks at 205 nm and 260 nm tell us only that the molecule has a conjugated
An infrared spectrum measures the absorption of energy as different bonds in the molecule vibrate in various ways.
It often gives information about the functional groups in a molecule.
The spectrum is usually a plot of the transmittance (or % transmission) of the light against the energy measured in wavenumbers (
The broad band from
The peaks at
The peak at
Mass spectroscopy is a technique that fragments a molecule into ions and sorts them based on their mass-to-charge (
The masses of the fragments often give us useful information on the structure of the molecule.
In a mass spectrogram, the relative abundance of each ion is plotted against its
The peak at mass 135 is the molecular ion (
Other major peaks are at
This tells you that you have very stable nitrogen-containing aromatic rings.
A sample is placed in a magnetic field and irradiated with a radiofrequency that causes the
Each atom absorbs an energy that depends on its environment in the molecule, so the spectrum tells us how many "different" carbon atoms are in the molecule.
The spectrum is a plot of signal intensity against the frequency measured in "ppm".
The 50 MHz