# Can someone help me with this question ??

So that means stuff at the bottom that runs the fastest is closes to the 5$end (if you are making GTCGAC and you incorporate as your first nucleotide a G that terminates the synthesis, then you get a glowing single nucleotide, G) So we read the 5$-3$sequence from the bottom up. 5'-ACTGAACTCGT-3$