How are restriction and DNA ligase enzymes used to insert a foreign gene into a plasmid?
A restriction enzyme is a DNA-cutting enzyme.and the ligase enzyme seals the gap between the molecules.
A restriction enzyme is a DNA-cutting enzyme that recognizes specific sites in DNA. Many restriction enzymes make cuts at or near their recognition sites which produce ends with a single-stranded overhang.
If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase.