Question

How much protein can a western blot detect?

Answer 1

depends on various factors. I have detected 2`mu`g of protein.

Explanation:

there are various factors which are involved in the process which include.

1. amount of protein in cell lysate
2. effectiveness/sensitivity of the antibodies
3. substrate sensitivity used for chemiluminescence

i have detected about 2`mu`g of a membrane protein in my cell lysate.

with purified protein i could detect approximate 1`mu`g.

but above three factors are crucial.