What is an elisa test?

1 Answer
Jun 2, 2018

ELISA stands for enzyme linked immunosorbent assay and is used to test /identify a substance with the help of antibodies and colour change.


In this process an unknown amount of antigen is affixed to a surface followed by the application of a specific antibody. The antibody binds to the antigen to form a complex. The antibody is co-valently linked to an enzyme. A substance that can convert to a detectable signal (like a colour change) is finally added. The visible signal indicates the quantity of antigens in the sample, which correlates to the amount of analyte present in the original sample.

ELISA tests are quick and can handle a large number of samples in parallel. They are thus a very popular choice for the evaluation of various research and diagnostic tools.

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