What is the role of lysis solution and heating the onion cell in waterbath for DNA extraction?

1 Answer
Mar 17, 2018

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Explanation:

It has been a while since I did DNA extraction from onion cells - it is a tearful process.

DNA is way on the inside. If you had a cell, and you were talking about the most "inner" part, that is where the DNA would be. Lysis solution is probably to break apart the cell wall/memebrane. Heating just helps this process (if you heat our cells, they come apart like a melting candle). Heat helps to disrupt the cell wall/membrane of the onion cell. Lysis solution probably helps to degrade any membranes the DNA is hold behind.

Heat and Lysis (lysis in biology generally means "cutting" - and hydrolysis is "cutting with water") help to make sure the DNA is fully removed from the cell by getting rid of all the parts of the cell that keep it intact.