# Question #470a1

##### 1 Answer

I made some assumptions, but here's how to do it.

**Step 1. Calculate the concentration of the original protein**

This comes from Beer's Law:

where

For proteins,

I will assume that your protein had

**Step 2. Calculate the concentration of the diluted protein**

You took 15.0 µL of the sample and diluted it with 15.0 µL of buffer to get 30.0 µL of solution.

You get the new concentration from a standard dilution calculation.

**Step 3. Calculate the mass of protein loaded on the gel**

You loaded 20.0 µL of the dilute solution onto the gel.