Question #006f1
1 Answer
Sep 9, 2016
Plate out a known volume on the correct media, and grow. Count the colonies.
Explanation:
Example calculation:
Plate out 50 µl
Counted colonies - 75
The 75 colonies come from 50 µl, hence in 1 ml there would be:
75 x 20 colonies (there are 20 lots of 50 µl in 1 ml) = 1,500
However, there was a 1 in 10 dilution at the start, so in total there would be 1,500 x 10 = 15,000 CFU/ml.
