A laboratory worker prepared a 1:200 dilution of whole blood. The diluted blood gave a count of 30 RBCs per hemocytometer square (100 µm × 100 µm). How many RBCs are in 1 mL of the diluted blood and in 1 mL of the whole blood?
Step 1. Calculate the volume of a hemocytomer square
So, there are 30 RBCs in
Step 2. Calculate the number of RBCs in a volume of
Step 3. Calculate the number of RBCs in 1 mL of whole blood
Your dilution factor is 200.
∴ In 1 mL of whole blood,