Can I combine a fragment of bacterial DNA cut with Hind III and a fragment of monkey DNA cut with Sma I? Why or why not?

1 Answer
Write your answer here...
Start with a one sentence answer
Then teach the underlying concepts
Don't copy without citing sources
preview
?

Answer

Write a one sentence answer...

Answer:

Explanation

Explain in detail...

Explanation:

I want someone to double check my answer

Describe your changes (optional) 200

2

This answer has been featured!

Featured answers represent the very best answers the Socratic community can create.

Learn more about featured answers

Feb 2, 2018

Answer:

Theoretically, yes, but difficult...

Explanation:

HinD III cuts 6-BP recognition sites:

5'-A#color(red)|#AGCTT-3'
3'-TTCGA#color(red)|#A-5'

leaving "sticky ends".

SMa1 also cuts 6 BP sites, but produces blunt ends:

5'-CCC#color(red)|#GGG-3'
3'-GGG#color(red)|#CCC-5'

Blunt ends can also be glued, but you would need to "fill in the gap" on the "sticky ends".

I know it has been done, as one of my colleagues did it, but it wasn't easy and prone to errors.
Also, the yield (success rate) is much lower than with sticky ends...

Was this helpful? Let the contributor know!
1500
Impact of this question
44 views around the world
You can reuse this answer
Creative Commons License