Question

Can someone help me with this plz?

Answer 1

See Below

Explanation:

Cutting with only EcoR1 will produce 2 fragments:
1st: 2270bp (3100-830)
2nd: 4130bp (6400-3100+830)

Cutting with BamH1 and EcoR1 together will produce 4 fragments:
1st. 830bp (830-0)
2nd: 1350bp (6400 - 5050)
3rd: 1950bp (5050-3100)
4th: 2270bp (3100-830).

Hope that helps.