How do restriction enzyme cut at specific sequences?
Restriction enzymes are used in the laboratory to cut DNA into smaller fragments. The specific restriction sites of DNA are generally palindromic sequences .
The cuts are always made at specific nucleotide sequences . Different restriction enzymes recognise and cut different DNA sequences.
Restriction enzymes work by shape to shape matching . It comes into contact with a DNA sequence with a shape that matches a part of the enzyme , called the restriction site. It then wraps around the DNA and causes a break in both the strands of the DNA molecule.
Each restriction enzymes recognises a different and specific recognition site or DNA sequence. Recognition sites are usually only 4 - 8 nucleotides long.
Restriction enzymes are basic tools for biotechnological research and are used for DNA cloning and DNA fingerprinting.
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