Question

How do you make a transgenic organism?

Answer 1

Genetic Engineering.

Explanation:

Basic outline:

• Take a bacterial plasmid. Cut out the portion of gene that you are interested to incorporate (into the plasmid, from another organism), using a restriction enzyme. The same restriction enzyme is used to cut the bacterial plasmid and using T4 DNA Ligase, incorporate the desired portion of DNA into the plasmid. The incorporated portion is called a transgene.

• Now, using a vector (bacteria/virus) which has a high multiplication rate; transport the transgene into the desired organism.

• The transgene replicates within the host cell or incorporates itself into the host DNA, and commands for the production of required protein synthesis.

• Your transgenic organism ( GMO ) is now ready for use!