Question

The presence of which enzyme is analysed via the oxidase assay? a) catalase b) glucose oxidase c) NADH oxidase d) cytochrome oxidase

Answer 1

Option `(d)` is correct.

Explanation:

Oxidase assay or oxidase test is particularly performed for detection of cytochrome c oxidase in `ETC`(electron transport chain) of bacteria. Basically four methods are used to test wether a specific bacterium is oxidase-positive or oxidase negative.

• Dry Filter Paper Method

• Wet Filter Paper Method

• Direct Plate Method

• Test tube Method

Bacteria having cytochrome oxidase give `color(purple)("purple")` colour at the end of each test. So purple colour is an indication of oxidase-positive bacteria.

Give a read to the link below to have detailed info about each of these methods:
https://microbiologyinfo.com/oxidase-test-principle-uses-procedure-types-result-interpretation-examples-and-limitations/

Hope it helps!