The presence of which enzyme is analysed via the oxidase assay? a) catalase b) glucose oxidase c) NADH oxidase d) cytochrome oxidase

1 Answer
Feb 15, 2018

Option #(d)# is correct.

Explanation:

Oxidase assay or oxidase test is particularly performed for detection of cytochrome c oxidase in #ETC#(electron transport chain) of bacteria. Basically four methods are used to test wether a specific bacterium is oxidase-positive or oxidase negative.

  • Dry Filter Paper Method

  • Wet Filter Paper Method

  • Direct Plate Method

  • Test tube Method

Bacteria having cytochrome oxidase give #color(purple)("purple")# colour at the end of each test. So purple colour is an indication of oxidase-positive bacteria.

Give a read to the link below to have detailed info about each of these methods:
https://microbiologyinfo.com/oxidase-test-principle-uses-procedure-types-result-interpretation-examples-and-limitations/

Hope it helps!