How do you measure concentration of reducing sugars?
One method is to do a colorimetric determination using Benedict's solution.
Benedict's solution is a deep-blue alkaline solution of stabilized
It oxidizes aldehyde groups to carboxylate ions, and the
Heating the unknown to about 95°C with Benedict's solution leads to the formation of a brick-red precipitate that indicates the presence of a reducing sugar.
You treat your unknown and a standard set of solutions containing known concentrations of glucose with standard Benedict's solution.
You remove the precipitate and measure the intensity of absorption in a colorimeter.
A calibration graph prepared from the glucose solutions enables you to determine the concentration of your unknown.
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