What is the difference between antibody capture ELISA and Sandwich ELISA?
1 Answer
Aug 19, 2016
As far as I know (and I stand to be corrected on this) there is no difference.
Explanation:
As far as I know "antibody capture" and "sandwich" ELISAs are the same.
In both cases a "capture" antibody is coated onto the ELISA plate (spare binding sites are blocked) before the addition of the sample. The antigen in the sample binds to the "capture" antibody, and then any unbound material is washed away. By using a "capture" antibody you effectively increase the amount of antigen present for detection.
You then proceed with a standard ELISA.