How can you isolate nuclei from cells?

1 Answer
Jan 8, 2018

When we want to isolate a nucleus from a cell then we talk about the process of enucleation.
For the process you need: a microscope, a suction pipette and a glass needle


This process is mostly used with oocytes, female egg cells.

  1. step
    We use a suction pipette (B) to hold the egg cell (A) and a glass needle (C) to remove the nucleus form the cell.

  2. step
    We push the glass needle through the zona pellucida that surrounds the egg cell. On the picture the glass needle is in the process of removing the nucleus.

  3. step #-># the result
    The egg cell's nucleus (A) is outside of the egg B.