Question

What are the limitations of PCR?

Answer 1

PCR is a technique used in molecular Biology to amplify a single copy/few copies of a segment of DNA, generating thousands to millions of copies of a particular DNA sequence.

Explanation:

One major drawback of PCR is a that prior information about the target sequence is necessary in order to generate the primers that will allow its selective amplification. Like all enzymes, DNA polymerase are also prone to error, which in turn causes mutations in the PCR fragments that are generated.