Question #2766f
1 Answer
Jan 30, 2015
There are 5.25 × 10⁷ cells/mL in the original sample.
Step 1. Find the volume of a box.
I assume that the depth of the box is 0.1 mm. Then
Note: 1 mL = 1 cm³ = 1000 mm³.
Step 2. Calculate the concentration of cells in a box.
Step 3. Correct for the dilution factor (DF).
Your original sample was 30 times more concentrated (DF = 30).
Original concentration = DF × concentration in slide box = 30 × 1.75 × 10⁶ cells/mL = 5.25 × 10⁷ cells/mL
The original sample contained 5.25 × 10⁷ cells/mL.