# How do you calculate dilution factor?

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You divide the final volume by the initial volume.

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**EXAMPLE 1**:

What is the dilution factor if you add a 0.1 mL aliquot of a specimen to 9.9 mL of diluent?

**Solution**:

You have diluted the sample by a factor of 100.

The dilution factor is often used as the denominator of a fraction.

For example, a

**EXAMPLE 2**:

How would you make 500 mL of a 1:250 dilution?

**Solution**:

Pipet 2.00 mL of your stock solution into a 500 mL volumetric flask.

Add diluent to the mark on the flask (you will have added about 498 mL of water).

You now have a 1:250 dilution of your original solution.

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