How do you calculate dilution factor?
You divide the final volume by the initial volume.
What is the dilution factor if you add a 0.1 mL aliquot of a specimen to 9.9 mL of diluent?
You have diluted the sample by a factor of 100.
The dilution factor is often used as the denominator of a fraction.
For example, a
How would you make 500 mL of a 1:250 dilution?
Pipet 2.00 mL of your stock solution into a 500 mL volumetric flask.
Add diluent to the mark on the flask (you will have added about 498 mL of water).
You now have a 1:250 dilution of your original solution.