Question

Question #4cfc3

Answer 1

The dilution factor wil be 65, or 1:65.

The dilution factor is expressed as the ratio between the final and the intial volumes of the solution.

`"DF" = V_("final")/V_("initial")`

The initial volume will be the `"0.1 mL"` supernatant sample you're using for the protein estimation. Now, the final volume will be equal to the sum of all the volumes you add to the test tube

`V_("final") = V_("supernatant") + V_("NaOH") + V_("reagent C") + V_("Folin")`

`V_("final") = "0.1 mL" + "0.9 mL" + "5 mL" + "0.5 mL" = "6.5 mL"`

This means that your dilution factor will be

`"DF" = V_("final")/V_("initial") = "6.5 mL"/"0.1 mL" = 65`, or `"1:65"`.

You've essentially added `"6.4 mL"` to your original `"1-mL"` sample to make the volume 65 times larger `->` you've performed a `"1:65"` dilution.

More on calculating dilution factors here:

http://socratic.org/questions/how-do-you-calculate-dilution-factor?source=search

http://socratic.org/questions/how-do-you-do-serial-dilution-calculations?source=search