Question #104c1

1 Answer
Feb 11, 2015

A 10X TBE solution is 10 times as concentrated as a 1X TBE solution, which means that you must dilute it 10-fold to get it to match a 1X TBE solution.

Since the solution will have various components of different molarities, #"X"# is used to illustrate a concentrated solution - 1X, 5X, 10X, 100X, and so on.

This is usefull when you're not concerned about the individual components of the solution, but rather about how concentrated or diluted a solution is.

So, you're basically dealing with a dilution calculation

#C_1V_1 = C_2V_2#

In you case, you'll get

#"10X TBE" * "10 mL" = "1X TBE" * V_("final")#

#V_("final") = "10X"/"1X" * "10 mL" = "100 mL"#

This will be the final volume of the solution, which means that the volume of buffer you must add is

#V_("buffer") = V_("final") - "10 mL" = 100 - 10 = "90 mL"#