What is the dilution factor if a 200 µL sample is diluted by adding 500 µL and then 300 µL of solvent?

1 Answer
Jul 16, 2016

Answer:

The dilution factor is 5.

Explanation:

The dilution factor #"DF"# is given by

#color(blue)(|bar(ul(color(white)(a/a) "DF" = V_f/V_i color(white)(a/a)|)))" "#

where

#V_f = "final volume" = "200 µL + 500 µL + 300 µL" = "1000 µL"#

#V_i = "sample volume" = "200 µL"#

#"DF" = (1000 cancel("µL"))/(200 cancel("µL")) = 5#

You have diluted the sample by a factor of 5.

Your #"DF"# is 5.

The volume of reagent does not affect the dilution factor in any special way. It simply contributes to the final volume.