Question #111d3
1 Answer
Aug 15, 2017
How about this?
Explanation:
You want each dilution to be 1:3 — 100 µL of solution + 200 µL of buffer = 300 µL.
For your last dilution, use 100 µL of solution, 150 µL of buffer, and 50 µL of bacteria.
Total volume = 300 µL, so this is still a 1:3 dilution.
