Restriction Enzymes

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Questions

• What is a restriction enzyme?
• Why must you use the same restriction enzyme on the cell dna and on the plasmid?
• Where do restriction enzymes come from in nature and what is their biological function?
• What determines where a restriction endonuclease "cuts" two DNA molecules at the same location?
• What type of organism produces restriction enzymes?
• Why are most restriction enzymes reactions performed at 37 degrees Celcius?
• Why are restriction enzymes kept in 50% glycerol solution?
• How do restriction enzyme cut at specific sequences?
• Do restriction enzymes cut both forward and backward?
• What is a biological function of restriction enzyme?
• If you have a restriction enzyme that cuts a piece of DNA at two recognition sites, how many DNA fragments would you see on a gel?
• From which organisms were restriction enzymes first isolated?
• What are the blunt ends in a DNA called when it is cut by a restriction enzyme?
• What is the function of restriction enzymes?
• What types of sequences do restriction enzymes cut?
• Do restriction enzymes cut both strands of a double stranded DNA molecule?
• How would you explain the mechanism of action of restriction enzymes?
• How does bacteria protect their own DNA against restriction enzymes?
• Would a restriction endonuclease cut messenger RNA?
• What is a biological function of restriction enzyme?
• How does a restriction enzyme limit or restrict the effect of a virus on a bacterial cell?
• Do restriction enzymes exist naturally in organisms?
• How are restriction enzymes used to make both recombinant DNA and transgenic organisms?
• Why is it more important for the restriction enzymes to recognise palindromic seqeunces?
• Why do most restriction enzyme cuts at palindromic sequence?
• What is a major source of restriction enzymes?
• What are the function of restriction enzymes in nature?
• Why are restriction enzymes important for DNA fingerprinting?
• Why are restriction enzymes important for recombinant DNA technology?
• What is the difference between single digestion and double digestion when discussing restriction enzymes?
• Why are restriction enzymes important for DNA fingerprinting?
• What benefit do bacteria gain by producing restriction enzymes?
• How do you choose which restriction enzymes to use when cloning a gene?
• How are restriction and DNA ligase enzymes used to insert a foreign gene into a plasmid?
• What role does gel electrophoresis play in the study of DNA?
• Question #31bc6
• Question #3beaf
• PBR322 is a plasmid having two restriction sites for EcoRI while T4 phage DNA has three restriction sites for it. These two DNA were treated with EcoRI and allowed to run on agarose gel. What kind of a pattern will be obtained on the gel?
• What enzyme is used to unwind DNA?
• Why is it essential that the same restriction enzyme be used to cleave (cut) the DNA of both organisms used to create a transgenic organism?